期刊
EMBO JOURNAL
卷 21, 期 6, 页码 1487-1496出版社
WILEY
DOI: 10.1093/emboj/21.6.1487
关键词
crystal structure; DNA complexes; helicase assembly; papillomavirus; replication-initiation
资金
- NCI NIH HHS [P01 CA013106, CA13106] Funding Source: Medline
- NIAID NIH HHS [AI46724, R01 AI046724] Funding Source: Medline
Initiation of DNA replication of the papillomavirus genome is a multi-step process involving the sequential loading of viral E1 protein subunits onto the origin of replication. Here we have captured structural snapshots of two sequential steps in the assembly process. Initially, an E1 dimer binds to adjacent major grooves on one face of the double helix; a second dimer then binds to another face of the helix. Each E1 monomer has two DNA-binding modules: a DNA-binding loop, which binds to one DNA strand and a DNA-binding helix, which binds to the opposite strand. The nature of DNA binding suggests a mechanism for the transition between double- and single-stranded DNA binding that is implicit in the progression to a functional helicase.
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