期刊
JOURNAL OF MEMBRANE BIOLOGY
卷 186, 期 2, 页码 81-88出版社
SPRINGER
DOI: 10.1007/s00232-001-0137-7
关键词
tight junctions; retinal pigment epithelium; blood-brain barrier; retina; junctions; epithelia
资金
- NEI NIH HHS [EY08694, EY00785] Funding Source: Medline
- NICHD NIH HHS [N01-HD-6-2915] Funding Source: Medline
During the development of chick retinal pigment epithelium (RPE), the permeability and selectivity of the epithelium's. tight junctions are continuously modulated. Overall paracellular permeability decreases, but selectivity increases. Because the claudin family of transmembrane proteins appears to provide the structural basis for selectivity, we examined the expression of claudins as a function of development in chick RPE. Degenerate primers were used with the reverse transcriptase-polymerase chain reaction (RT-PCR) to obtain complete sequences of chick claudins 3 and 5. Northern blotting and semiquantitative RT-PCR demonstrated that claudin 5 was expressed in RPE, but claudin 3 was expressed only in the choroid layer of the eye. Northern blotting, semiquantitative RT-PCR and immunoblotting demonstrated that the expression of claudin 5 was transient, with peak levels of expression between embryonic days 10 and 14. Primary cultures were used to demonstrate that I, actors secreted by the neural retina induced the expression of claudin 5 nearly 3-fold if RPE was isolated from embryonic day 7 embryos. There was little effect if RPE was isolated from embryonic day 14. The upregulation of claudin 5 correlates with permeability changes that occur during the intermediate stage of RPE development. Interestingly, claudin 5 must be replaced during the late stage of development when the number and complexity of tight junctional strands increases. This would imply more changes in selectivity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据