期刊
GENES & DEVELOPMENT
卷 16, 期 6, 页码 743-752出版社
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.967302
关键词
HATs; HDACs; transcription; histone acetylation; chromatin; gene regulation
资金
- NIGMS NIH HHS [GM30186, R37 GM030186, R01 GM030186, R01 GM053720, GM53720] Funding Source: Medline
DNA-binding activators and repressors recruit histone acetylases and deacetylases to promoters, thereby generating localized domains of modified histones that influence transcriptional activity. At the end of a transcriptional response, alterations in histone acetylation status are reversed, but the dynamics of this process are poorly understood. Here, we recruit histone deacetylases and acetylases to a well-defined yeast promoter in a regulated manner. Following dissociation of the recruiting protein from the promoter, targeted deacetylation and acetylation are reversed with rapid, yet distinct, kinetics. Reversal of targeted deacetylation occurs within 5-8 min, whereas reversal of targeted acetylation is more rapid, taking 1.5 min. These findings imply that untargeted, globally acting enzymes generate a highly dynamic equilibrium of histone acetylation and deacetylation reactions across chromatin. Targeted acetylases and deacetylases can locally perturb this equilibrium, yet once they are removed, the global activities mediate a rapid return to the steady-state level of histone acetylation. Our results also indicate that TBP occupancy depends on the presence of the activator, not histone acetylation status.
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