4.7 Article

The role of ARK in stress-induced apoptosis in Drosophila cells

期刊

JOURNAL OF CELL BIOLOGY
卷 156, 期 6, 页码 1077-1087

出版社

ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.20112068

关键词

apoptosis; ARK; DIAP; mitochondria; Drosophila

资金

  1. NCI NIH HHS [CA69381, P01 CA069381] Funding Source: Medline
  2. NIAID NIH HHS [AI40646, R01 AI040646] Funding Source: Medline

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The molecular mechanisms of apoptosis are highly conserved throughout evolution. The homologs of genes essential for apoptosis in Caenorhabditis elegans and Drosophila melanogaster have been shown to be important for apoptosis in mammalian systems. Although a homologue for CED-4/apoptotic protease-activating factor (Apaf)-1 has been described in Drosophila, its exact function and the role of the mitochondrial pathway in its activation remain unclear. Here, we used the technique of RNA interference to dissect apoptotic signaling pathways in Drosophila cells. Inhibition of the Drosophila CED-4/Apaf-1 -related killer (ARK) homologue resulted in pronounced inhibition of stress-induced apoptosis, whereas loss of ARK did not protect the cells from Reaper- or Grim-induced cell death. Reduction of DIAP1 induced rapid apoptosis in these cells, whereas the inhibition of DIAP2 expression did not but resulted in increased sensitivity to stress-induced apoptosis; apoptosis in both cases was prevented by inhibition of ARK expression. Cells in which cytochrome c expression was decreased underwent apoptosis induced by stress stimuli, Reaper or Grim. These results demonstrate the central role of ARK in stress-induced apoptosis, which appears to act independently of cytochrome c. Apoptosis induced by Reaper or Grim can proceed via a distinct pathway, independent of ARK.

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