期刊
TALANTA
卷 56, 期 5, 页码 867-874出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(01)00660-9
关键词
chemically modified DNA; osmium tetroxide; voltammetry; pyrolytic graphite electrode; DNA labeling
DNA and synthetic polynucleotides modified with a complex of osmium tetroxide with 2,2'-bipyridine (Os,bipy) produce specific voltammetric signals at pyrolytic graphite electrodes. Based on a sufficient potential separation between the peaks of Os,bipy-modified DNA (DNA-Os,bipy) and of free Os,bipy, and using an adsorptive transfer stripping voltammetric procedure involving extraction of free Os,bipy from the electrode by chloroform, DNA-Os,bipy can be determined in an excess of the free reagent. Under certain conditions, 140 pg of DNA-Os,bipy can be detected after a 5 min accumulation period. This analysis displays a more favorable sensitivity and a better selectivity for DNA structure than oxidation of DNA guanine moieties, and offers detection of osmium DNA markers at carbon electrodes. (C) 2002 Elsevier Science B.V. All rights reserved.
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