期刊
METHODS
卷 26, 期 4, 页码 341-347出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1046-2023(02)00040-3
关键词
apoptosis; mitochondria; cytochrome c; membrane potential; fluorescence microscopy; flow cytometry; subcellular fractionation; Bax; green fluorescent protein
Mitochondria play a central role in programmed cell death through the release of cytochrome c and other proapoptotic factors. Fluorescence microscopy is used to visualize cytochrome e translocation and loss of mitochondrial membrane potential. Flow cytometry can also be used to measure mitochondrial membrane potential. Cytochrome c content in cytosol and mitochondria can be determined by immunoblotting after subcellular fractionation or selective permeabilization with digitonin. Isolated mitochondria can be used to study the mechanism of cytochrome c release. This article summarizes some of the more widely used methods to assess mitochondrial alterations in apoptosis. (C) 2002 Elsevier Science (USA). All rights reserved.
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