期刊
PLANT JOURNAL
卷 30, 期 2, 页码 237-245出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-313X.2001.01276.x
关键词
secondary ion mass spectrometry; SIMS; secondary electron microscopy; SEM; ion micro-analysis; plant; wheat seed
An imaging secondary ion mass spectrometry system has been developed that allows the distribution of elements or ions to be superimposed on an image of the plant cell or tissue generated by ion-induced secondary electrons. This system has been evaluated by analysing the aleurone and sub-aleurone cells of mature wheat grain, showing high spatial resolution (100-200 nm) images of O- , PO2 (-) , Mg+ , Ca+ , Na+ and K+ within the phytate granules of the aleurone, with CN- being diagnostic for proteins and C-2 (-) being diagnostic for starch in the starchy endosperm cells. This system should provide improved localization of elements in a range of other plant systems.
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