期刊
SOIL SCIENCE AND PLANT NUTRITION
卷 48, 期 2, 页码 271-277出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00380768.2002.10409200
关键词
anion channels; barley; nitrate; xylem loading
The effects of anion channel blockers, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and anthracene-9-carboxylic acid (A-9-C), on the uptake and xylem transport of nitrate were investigated in intact barley (Hordeum vulgare L.) seedlings using (NO3-)-N-13 and (NO3-)-N-15 as tracers. The seedling roots were pretreated with either blocker for 1 h and then (NO3-)-N-13 or (NO3-)-N-15 was supplied to the medium. Real-time images of N-13 accumulation in shoots were monitored during the first 30 min using Positron Emitting Tracer Imaging Systems (PETIS). The radioactivity in the shoots of the plants treated with either blocker was about half of that in the shoots of the control plants. Analysis of the distribution of N-13 in whole seedlings using a Bioimaging Analyzer System (BAS) showed a N-13-accumulation in both shoots and roots of the control plants but reduced N-13-levels in the shoots of the plants treated with either blocker. Nitrate concentrations in the xylem sap were significantly reduced by the application of either blocker while the net uptake of (NO3-)-N-15 was not or slightly influenced by the treatment with blockers. The translocation and uptake of chloride were also significantly reduced by the treatment with A-9-C but not with DIDS. These results suggested that anion channels contributed to xylem loading of nitrate in barley plants.
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