期刊
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
卷 22, 期 4, 页码 413-419出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/10799900252952190
关键词
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The function of the common carp, Cyptinus carpio L., interleukin-1beta (IL-1beta) gene was studied by DNA injection. To investigate the immune responses to IL-1beta, a plasmid construct of cytomegalovirus (CMV)-driven carp IL-1beta was injected into the epaxial muscle of carp. IL-1beta protein expressed in serum on 1, 3, and 5 days after plasmid injection was quantified by ELISA and immunohistochemistry. IL1-beta gene injection increased proliferation of the lymphocytes by phytohemagglutinin (PRA). Macrophage functions, such as production of superoxide anion and phagocytosis, also were stimulated by IL-1beta gene injection. Moreover, an increase in resistance to Aeromonas hydrophila infection was recorded in IL-1beta-injected fish compared with control fish. Thus, the cloned homolog of IL-1beta from carp has all the functional similarities to the mammalian IL-1beta gene.
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