期刊
NEUROPHARMACOLOGY
卷 42, 期 5, 页码 653-661出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0028-3908(02)00019-9
关键词
nicotine; GABA; transmitter release; locus coeruleus; ontogeny
资金
- NIDA NIH HHS [DA 10612] Funding Source: Medline
The primary role of nicotinic acetylcholine receptors in adult and developing brain is to modulate neurotransmission. Using in vitro neurotransinitter release, we have examined mechanisms underlying nicotine-induced [H-3]norepinephrine release from developing and adult rat hippocampus. At birth, nicotine significantly stimulated hippocampal [H-3]norepinephrine release with a monotonic increase in maximal drug effect over the first ten postnatal days. No developmental changes in agonist or antagonist potency were observed, Comparison of synaptosomal and slice preparations, as well as examination of the effects of tetrodotoxin, indicated that at least two nicotinic acetylcholine receptor populations regulated [H-3]norepinephrine release from neonatal and adult hippocampus; one localized on noradrenergic terminals, the other on adjacent cells. To further characterize the indirect mechanism of nicotine action ill the adult, we examined the effects of pharmacological blockade of various neurotransmitter systems that provide excitatory input to hippocampal noradrenergic terminals. Whereas glutamate and muscarinic receptor blockade was ineffective, the GABA-A receptor antagonists, bicuculline and picrotoxin, inhibited the indirect component of nicotine-mediated [3H]norepinephrine release. Furthermore, pentobarbital, an allosteric effector at GABA-A receptors, potentiated the effect of submaximal concentrations of nicotine. These findings are consistent with the hypothesis that nicotine-induced GABA release serves as an additional stimulus for [H-3]norepinephrine secretion within rat hippocampus. (C) 2002 Elsevier Science Ltd. All rights reserved.
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