期刊
IMMUNOLOGY AND CELL BIOLOGY
卷 80, 期 2, 页码 156-163出版社
BLACKWELL PUBLISHING ASIA
DOI: 10.1046/j.1440-1711.2002.01071.x
关键词
CD8 T lymphocyte; cytotoxic T lymphocyte; herpes simplex virus; major histocompatibility complex class I; T cell receptor; thymus; transgenic T cell receptor
To better understand the T cell-mediated processes involved in the immune response to herpes simplex virus type 1 (HSV-1) infection, two HSV-specific T cell receptor (TCR) transgenic mouse lines were produced. These mice (gBT-I.1 and gBT-I.3) are MHC class I-restricted and specific for the immunodominant peptide from HSV glycoprotein B (gB), gB(498-505) . Although derived from the same clone, the mice differ in the chromosomal location of the TCR transgenes and show marked differences in TCR alpha/beta expression on both CD4(+) and CD8(+) cells in the thymus. Despite this, peripheral CD8(+) T cells from both mice express equally high levels of the transgenic TCR and bind the K-b gB(498-505) tetramer to the same degree. In concordance with this, both were shown to respond equally well in vitro upon stimulation with the gB(498-505) peptide or HSV-infected cells. These data show that selection of broadly equivalent peripheral T-cell subsets can occur in the presence of distinctly different thymic T-cell subsets.
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