期刊
ANNALS OF BOTANY
卷 89, 期 4, 页码 491-497出版社
OXFORD UNIV PRESS
DOI: 10.1093/aob/mcf054
关键词
protoplast fusion; genetic transformation; green fluorescent protein; GFP; woody plant; vital marker; Citrus reticulata; Citrus sinensis; Poncirus trifoliata; Agrobacterium tumefaciens
Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants, It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants. (C) 2002 Annals of Botany Company.
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