期刊
JOURNAL OF BACTERIOLOGY
卷 184, 期 7, 页码 1905-1915出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.184.7.1905-1915.2002
关键词
-
类别
资金
- NIGMS NIH HHS [R01 GM045404, GM45404] Funding Source: Medline
Various mutant strains were used to examine the regulation and metabolic control of the Calvin-Benson-Bassham (CBB) reductive pentose phosphate pathway in Rhodobacter capsidatus. Previously, a ribulose 1,5-bisphosphate carboxylase/oxygenase, (RubisCO)-deficient strain (strain SBI/II) was found to show enhanced levels of cbb(I) and cbb(II) promoter activities during photoheterotrophic growth in the presence of dimethyl sulfoxide. With this strain as the starting point, additional mutations were made in genes encoding phosphoribulokinase and transketolase and in the gene encoding the LysR-type transcriptional activator, CbbR(II). These strains revealed that a product generated by phosphoribulokinase was involved in control of CbbR-mediated cbb gene expression in SBI/II. Additionally, heterologous expression experiments indicated that Rhodobacter sphaeroides CbbR responded to the same metabolic signal in R. capsulatus SBI/II and mutant strain backgrounds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据