期刊
JAPANESE JOURNAL OF PHYSIOLOGY
卷 52, 期 2, 页码 141-147出版社
CENTER ACADEMIC PUBL JAPAN
DOI: 10.2170/jjphysiol.52.141
关键词
okadaic acid; smooth muscle; skinned preparation
类别
The influences of cell membrane permeabilization (skinning) on the okadaic acid-induced inhibition of vascular smooth muscle contraction were studied in guinea pig hepatic portal vein. Pretreatment by 1 muM okadaic acid in the absence of Ca2+ suppressed subsequent submaximal Ca2+-induced contraction in preparations permeabilized with Staphylococcus aureus alpha-toxin or beta-escin, but not in those treated with saponin or Triton X-100. The SDS-PAGE of elutants from the preparation suggests that the loss of the inhibitory effect of okadaic acid in preparations skinned with saponin or Triton X-100 results from the leakage of some cellular components with a molecular mass of 67 to 200 kDa.
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