期刊
JOURNAL OF ARTHROPLASTY
卷 17, 期 3, 页码 335-346出版社
CHURCHILL LIVINGSTONE INC MEDICAL PUBLISHERS
DOI: 10.1054/arth.2002.30416
关键词
osteoclast; macrophage; wear particles; aseptic loosening; ceramics; tumor necrosis factor (TNF)-alpha; reactive oxygen species (ROS); superoxide; nitric oxide
类别
资金
- NIAMS NIH HHS [AR44501, AR39740, AR44046] Funding Source: Medline
- NIDCR NIH HHS [DE11082] Funding Source: Medline
Wear of orthopaedic implants generates particles capable of inducing bone resorption and aseptic loosening of the implant. The present study shows the combined effect of particles and cell activation on macrophage (THP-1) and osteoclast (HD-11EM) release of reactive oxygen and nitrogen species, providing insight into mechanisms that can lead to osteolysis. In the absence of cell activation, exposure of either cell type to submicron zirconia or latex particles did not elicit an increase in reactive oxygen and nitrogen species production. Suboptimal stimulation with 4beta-phorbol-12-myristate-13-acetate (PMA) plus particles resulted in a synergistic release of superoxide (O-2(-)), however, and a low-level production of nitric oxide (.) by THP-1 macrophages. Similarly, particle stimulation of tumor necrosis factor-alpha-activated THP-1 cells increased O-2(-) release. Our findings show the synergistic effect of cell activation and wear particles on O-2(-) production by activated macrophages and osteoclasts, suggesting O-2(-) involvement in mediating osteolysis.
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