期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 262, 期 1-2, 页码 5-19出版社
ELSEVIER
DOI: 10.1016/S0022-1759(02)00009-1
关键词
tandem mass spectrometry; electrospray ionisation; MHC class I peptides; tumour epitopes; peptide purification and sequencing
Electrospray ionisation (ESI) mass spectrometry (MS) has been used extensively for the detection of peptides presented by major histocompatibility complex (MHC) molecules, This review focuses on the optimisation of electrospray mass spectrometry and the use of tandem mass spectrometry to sequence MHC class I peptides, We review the isolation of MHC class I peptides from the surface of cells with particular reference to tumour cells. In addition, we also discuss the advantages and disadvantages of the methods available to concentrate and fractionate the peptides prior to analysis by electrospray mass spectrometry. (C) 2002 Elsevier Science B,V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据