Chiral CE separation of warfarin in albumin containing samples

A capillary zone electrophoresis method with ultraviolet (UV) absorbance detection for the chiral separation of warfarin enantiomers using highly sulfated beta-cyclodextrin (beta-CD) was developed and optimized. Enantiomeric separation of warfarin was characterized by high resolution and efficiency. The optimized electrophoretic conditions were subsequently applied to the analysis of warfarin extracted from spiked human serum albumin samples. This assay showed acceptable precision, with linearity in the warfarin enantiomer concentration range of 0.1-25 mg/l. The limits of detection (LOD) and quantitation (LOQ) evaluated as warfarin enantiomer concentrations in the serum samples were 0.05 and 0.15 mg/l, respectively, for each warfarin enantiomer. (C) 2002 Elsevier Science B.V. All rights reserved.