期刊
EMBO JOURNAL
卷 21, 期 7, 页码 1545-1554出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/21.7.1545
关键词
guanylate cyclase-activating proteins; paired flash electroretinograms; rod photoreceptors; single cell recordings; visual transduction
资金
- NEI NIH HHS [EY 08061, EY 07001, EY 04446, EY 11850, EY 02520, F32 EY007001, R01 EY008061, EY 0248, T32 EY007001, EY 08123, R01 EY011850, R01 EY004446, P30 EY002520, R01 EY008123] Funding Source: Medline
Visual transduction in retinal photoreceptors operates through a dynamic interplay of two second messengers, Ca2+ and cGMP. Ca2+ regulates the activity of guanylate cyclase (GC) and the synthesis of cGMP by acting on a GC-activating protein (GCAP). While this action is critical for rapid termination of the light response, the GCAP responsible has not been identified. To test if GCAP1, one of two GCAPs present in mouse rods, supports the generation of normal flash responses, transgenic mice were generated that express only GCAP1 under the control of the endogenous promoter. Paired flash responses revealed a correlation between the degree of recovery of the rod a-wave and expression levels of GCAP1 In single cell recordings, the majority of the rods generated flash responses that were indistinguishable from wild type. These results demonstrate that GCAP1 at near normal levels supports the generation of wild-type flash responses in the absence of GCAP2.
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