期刊
FEBS LETTERS
卷 516, 期 1-3, 页码 161-163出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(02)02533-4
关键词
tol-pal operon; thioesterase; Haemophilus influenzae; YbgC; bacterial cell envelope; peptidoglycan
资金
- NIGMS NIH HHS [GM28688] Funding Source: Medline
This paper examines the catalytic function of the protein YbgC, encoded by the ybgC gene of the tol-pal gene cluster in Haemophilus influenzae. The YbgC protein, a homologue of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-coenzyme A thioesterase, conserves the active site Asp residue associated with thioesterase activity. The H. influenzae ybgC gene was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and tested for thioesterase activity towards acyl-CoA and acyl-N-acetylcysteamine thioesters. The YbgC protein catalyzes the hydrolysis of short chain aliphatic acyl-CoA thioesters, while the D18N YbgC mutant protein (prepared to serve as a control) does not. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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