期刊
ORGANIC LETTERS
卷 4, 期 8, 页码 1395-1398出版社
AMER CHEMICAL SOC
DOI: 10.1021/ol0257225
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资金
- NIAID NIH HHS [AI 41598] Funding Source: Medline
[GRAPHICS] Cycloartenol synthase converts oxidosqualene to cycloartenol, a pentacyclic isomer of the animal and fungal sterol precursor lanosterol. We used directed evolution to find cycloartenol synthase residues that affect cyclopropyl ring formation, selecting randomly generated cycloartenol synthase mutants for their ability to genetically complement a yeast strain lacking lanosterol synthase. To increase the likelihood of finding novel mutations, the little-studied Dictyostelium discoideum cycloartenol synthase was used for the mutagenesis. Several catalytically important residues were identified.
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