期刊
FEMS MICROBIOLOGY LETTERS
卷 210, 期 1, 页码 55-60出版社
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6968.2002.tb11159.x
关键词
transcription activation; cAMP receptor protein; RNA polymerase alpha subunit; CRP-dependent promoter
类别
The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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