期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 99, 期 9, 页码 6204-6209出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.092154599
关键词
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资金
- NIAID NIH HHS [R21 AI 494897] Funding Source: Medline
- NIGMS NIH HHS [R01 GM39458, R01 GM039458] Funding Source: Medline
We have developed an alternative to transgenesis for producing antigen-specific T cells in vivo. in this system, clonal naive T cells with defined antigen specificity are generated by retrovirus-mediated expression of T cell antigen receptor cDNAs in RAG1-deficient murine hematopoietic precursor cells. These T cells can be stimulated to proliferate and produce cytokines by exposure to antigen in vitro, and they become activated and expand in vivo after immunization. IL-2-deficient T cells generated by this technique show decreased proliferation and cytokine production, both of which can be rescued by exogenous addition of this growth factor. Thus, retrovirus-mediated expression of T cell antigen receptor cDNAs in hematopoietic precursor cells permits the rapid and efficient analysis of the life history of antigen-specific T cells in different genetic backgrounds and may allow for the long-term production of antigen-specific T cells with different functional properties for prophylactic and therapeutic purposes.
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