期刊
JOURNAL OF MEDICAL VIROLOGY
卷 67, 期 1, 页码 41-46出版社
WILEY-LISS
DOI: 10.1002/jmv.2190
关键词
RT-PCR; immunostaining; TNF-alpha; IL-4; hemorrhagic fever
类别
资金
- NIAID NIH HHS [R01 AI30624, P01 AI34533] Funding Source: Medline
Plasma leakage in dengue hemorrhagic fever (DHF) is associated with elevated plasma levels of cytokines. To define further the contribution of immune activation to DHF and the source of cytokines, we analyzed the production of cytokines in peripheral blood mononuclear cells (PBMC) obtained from children with dengue, using RT-PCR and immunostaining. Tumor necrosis factor-alpha (TNF-alpha) and TNF-beta expression was detected in all samples by PCR and in < 50% of samples by immunostaining. Interferon-gamma (IFN-gamma) expression was detected in < 50% of samples by either method. Interleukin-2 (IL-2) and IL-4 expression was detected in a few samples by immunostaining but was not detectable by PCR. We found greater expression of TNF-α and IL-4 in DHF than in dengue fever or other (non-dengue) febrile illnesses. These results support the model of immunopathogenesis of DHF. However, low levels of cytokine expression in PBMC suggest that cellular activation in tissues may contribute to high serum cytokine levels in DHF. © 2002 Wiley-Liss, Inc.
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