期刊
BLOOD
卷 99, 期 9, 页码 3454-3457出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood.V99.9.3454
关键词
-
类别
资金
- NHLBI NIH HHS [P01 HL-55435] Funding Source: Medline
- NIDDK NIH HHS [DKS7199] Funding Source: Medline
- PHS HHS [R01 A130389] Funding Source: Medline
Ex vivo proliferation of hematopoietic stem cells (HSCs) Is Important for cellular and gene therapy but Is limited by the observation that HSCs do not engraft as they transit S/G(2)/M. Recently Identified candidate Inhibitors of human HSC cycling are transforming growth factor-beta(1) (TGF-beta(1)) and stroma-derived factor-1 (SDF-1). To determine the ability of these factors to alter the transplantability of human HSCs proliferating in vitro, lincord blood cells were first cultured for 96 hours In serum-free medium containing Flt3 ligand, Steel factor, interieukin-3, Interleukin-6, and granulocyte colony-stimulating factor. These cells were then transferred to medium containing Steel factor and thrombopoietin with or without SDF-1 and/or TGF-beta(1) for 48 hours. Exposure to SDF-1 but not TGF-beta(1) significantly increased (> 2-fold) the recovery of HSCs able to repopulate nonobese diabetic/severe combined immunodeficiency mice. These results suggest new strategies for improving the engraftment activity of HSCs stimulated to proliferate ex vivo. (C) 2002 by The American Society of Hematology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据