期刊
JOURNAL OF BIOMOLECULAR NMR
卷 23, 期 1, 页码 63-67出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1015397305148
关键词
N-15 spin relaxation; protein dynamics; slow motional modes; supercooled water; ubiquitin
The impact of studying protein dynamics in supercooled water for identifying slow motional modes on the mus time scale is demonstrated. Backbone N-15 spin relaxation parameters were measured at -13 degreesC for ubiquitin, which plays a central role for signaling proteolysis, cellular trafficking and kinase activation in eukaryotic organisms. A hitherto undetected motional mode involving Val 70 was found, which may well play an important role for ubiquitin recognition. The measurement of rotating frame N-15 relaxation times as a function of the spin-lock field allowed determination of the correlation time of this motional mode, which would not have been feasible above 0 degreesC.
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