Sensitivity of Eleusine indica callus to Trifluralin and amiprophosmethyl in correlation with the binding of these compounds to tubulin

The sensitivity of calluses derived from susceptible and resistant goosegrass (Eleusine indica (L.) Gaertn.) biotypes to dinitroaniline herbicides, which disrupt inter-phase and mitotic-spindle microtubules. was evaluated. A callus culture derived from the resistant biotype retained resistance to both trifluralin (dinitroaniline herbicide) and amiprophosmethyl (phosphorothioamidate herbicide). The site for the interaction between a-tubulin subunit and dinitroaniline or phosphorothioamidate herbicides was identified by computer simulation. A correlation was found between the level of callus sensitivity to herbicide tested and the pattern of herbicide interaction with alpha-tubulin.