期刊
PLANT JOURNAL
卷 30, 期 4, 页码 403-413出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-313X.2002.01296.x
关键词
pectin; fungal eRGL; Solanum tuberosum; transgenic plant; cell wall modification; immunolabelling
Rhamnogalacturonan (RG) I is a branched pectic polysaccharide in plant cell walls. Rhamnogalacturonan lyase (e RGL) from Aspergillus aculeatus is able to cleave the RG I backbone at specific sites. Transgenic potato (Solanum tuberosum L.) plants were made by the introduction of the gene encoding e RGL, under the control of the granule-bound starch synthase promoter. The e RGL protein was successfully expressed and translated into an active form, demonstrated by e RGL activity in the tuber extracts. The transgenic plants produced tubers with clear morphological alterations, including radial swelling of the periderm cells and development of intercellular spaces in the cortex. Sugar compositional analysis of the isolated cell walls showed a large reduction in galactosyl and arabinosyl residues in transgenic tubers. Immunocytochemical studies using the LM5 (galactan) and LM6 (arabinan) antibodies also showed a large reduction in galactan and arabinan side-chains of RG I. Most of the remaining LM5 epitopes were located in the expanded middle lamella at cell corners of e RGL tubers, which is in contrast to their normal location in the primary wall of wild type tubers. These data suggest that RG I has an important role in anchoring galactans and arabinans at particular regions in the wall and in normal development of the periderm.
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