Borna disease in a dog in Japan

Borna disease (BD) was diagnosed in a 3-year-old male Welsh corgi suffering from a severe and acute progressive disorder of the central nervous system. Histopathologically, neuronal lesions were characterized by a non-suppurative encephalomyelitis dominated by large perivascular cuffs consisting of lymphocyeds, macrophages and plasma cells; also present were inflammatory cell infiltrates in the neural parenchyma, neuronophagia and focal gliosis.Strong immunolabelling with BD virus (BDV) p40 antibody was diffusely distributed in the cytoplasm of small and large neurons in areas of the brain with and without inflammatory distributed in the cytoplasm of small and large neurons in areas of the brain with and without inflammatory changes, and also in the spinal cord. Positive hybridizaton signals with BDV p40 sense and antisense riboprobes were seen in the nucleus and cytoplasm of the neurons throughout the whole brain and spinal cord. BDV p24 RNA in formalin-fixed brain tissue was detected by reverse transcriptase (RT)-nested polymerase chain reaction (PCR). BDV p24 RNA-positive signals were detected in the temporal lobe. Thisl is the first report of spontaneous canine BD in Japan. (C) 2002 Published by Elsevier Science Ltd.