期刊
PLANT MOLECULAR BIOLOGY
卷 49, 期 2, 页码 187-197出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1014962923278
关键词
beta-expansins; pistil-specific expression; pollen-allergens; pollination
Using differential screening we isolated a pistil-specific cDNA clone corresponding to a 1.2 kb mRNA and encoding a 32.5 kDa protein. The amino acid sequence shared similarity with that of group-I grass pollen allergens, which are known to have expansin activity. This clone, which later showed to share homology also with beta-expansins, was named PPAL. The PPAL mRNA was specifically expressed in the secretory zone of the stigma and in the epidermal layer of the placenta. The accumulation level of the transcript increased during pollination, and the protein was secreted in the stigmatic exudate of the tobacco flower. We suggest here that PPAL is a new expansin, acting as a cell-wall-loosening agent during pollination.
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