Assessing bactericidal properties of materials:: the case of metallic surfaces in contact with air

A new method for assessing bactericidal properties of metallic materials, soiled by aerosol, was developed and applied to stainless steel in conditions close to reality. The airborne bacteria survival on different stainless steel grades and massive copper is presented here. The investigating bacterium was Enterococcus faecalis, which is a well-known contaminant strain in the indoor environments. It was observed that the bacterial aerosol lethality increased proportionally with the relative humidity (RH) of the environment. A significant difference in survival rate was measured depending on the tested supports, the greatest lethality being observed on clean massive copper. Moreover. the addition of nutrients on metallic surfaces, even in small quantities, was enough to ensure the revival of quiescent microorganisms. (C) 2002 Elsevier Science B.V. All rights reserved.