期刊
ANNALS OF SURGICAL ONCOLOGY
卷 9, 期 4, 页码 364-370出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1007/BF02573871
关键词
CEA-mRNA; RT-PCR; circulating tumor cells; hematogenous metastasis; liver metastasis; biliary-pancreatic cancer
Background: Circulating tumor cells in the blood were frequently detected by reverse transcription-polymerase chain reaction during operation in patients with biliary-pancreatic cancer. We investigated the relationship between circulating tumor cells during operation and hematogenous metastases. Methods: Blood samples from 67 patients with biliary-pancreatic cancer were obtained from the portal vein, peripheral artery, and superior vena cava during operation. After total RNA was extracted from each blood sample, carcinoembryonic antigen (CEA)-specific reverse transcription-polymerase chain reaction was performed. Results: Intraoperative CEA-messenger RNA (mRNA) expression was detected in the blood of 32 (47.8%) of 67 patients with biliary-pancreatic cancer, although it was not detected in the blood obtained from 20 healthy volunteers or 15 patients with benign disease of the biliary pancreas. The incidence (37.5%) of hematogenous metastases after surgery in the CEA-mRNA-positive group (n = 32) was significantly higher than that (11.4%) in the negative group (n = 35 P = .01). In stage I, II, and III patients. survival of the CEA-mRNA-positive group was significantly worse compared with that of negative group (P = .03). Conclusions: Intraoperative molecular detection of circulating tumor cells in patients with biliary-pancreatic cancer relates to a high risk of hematogenous metastasis and is associated with unfavorable prognosis even after curative resection.
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