期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
卷 60, 期 2, 页码 241-251出版社
JOHN WILEY & SONS INC
DOI: 10.1002/jbm.10073
关键词
bone regeneration; transforming growth factor-beta; recombinant proteins; osteoinduction; porous poly(propylene fumarate); scaffolds
资金
- NIAMS NIH HHS [R01-AR44381] Funding Source: Medline
- NIDCR NIH HHS [R01-DE13031, R01-DE13740] Funding Source: Medline
This study determined the bone growth into pretreated poly(propylene fumarate) (PPF) scaffolds implanted into a subcritical size, ribbit cranial defect. PPF scaffolds were constructed by using a photocrosslinking-porogen leaching technique. These scaffolds were then either prewetted (PPF-Pw), treated with RF glow-discharge (PPF-Gd), coated with fibronectin (PPF-Fn), or coated with rhTGF-beta(1) (PPF-TGF-beta(1)). One of each scaffold type was then placed into the cranium of nine rabbits. The rabbits were sacrificed after 8 weeks, and the scaffolds were retrieved for histological analysis. The most bone formation was present in the PPF-TGF-beta(1) implants; the newly formed bone had a trabecular appearance together with bone marrow-like tissue. Little or no bone formation was observed in implants without rhTGF-beta(1). These histological findings were confirmed by image analysis. Bone surface area, bone area percentage, pore fill percentage, and pore area percentage were significantly higher in the rhTGF-beta(1)-coated implants than in the noncoated implants. No statistical difference was seen between the PPF-Fn, PPF-Pw, or PPF-Gd scaffolds for these parameters. Quadruple fluorochrome labeling showed that in PPF-TGF-beta(1) implants bone formation mainly started in the interior of a pore and proceeded toward the scaffold. We conclude that (a) PPF-TGF-beta(1) scaffolds can indeed adequately induce bone formation in porous PPF, and (b) PPF scaffolds prepared by the photocrosslinking-porogen leaching technique are good candidates for the creation of bone graft substitutes. (C) 2002 John Wiley Sons, Inc.
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