期刊
MECHANISMS OF DEVELOPMENT
卷 113, 期 2, 页码 175-179出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(02)00016-3
关键词
cardiac development; cell migration; chicken; cushion cells; heart development; endocardium; epithelial-mesenchymal transformation; immunocytochemistry; in situ hybridization; matrix metalloproteinase-2; matrix metalloproteinase; myocardium; proteases; septation; tissue inhibitor of metalloproteinase-2; tissue inhibitor of metalloproteinase-3; tissue remodeling
Matrix metalloproteinases (MMPs) mediate cell migration and tissue remodeling and a-re important in cardiac development. We examined the expression patterns of two MMP inhibitors, tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-3, during critical stages of cardiac development, Both TIMP-2 and TIMP-3 mRNA were expressed in the endocardium prior to and during early cushion cell formation. TIMP-2 was continually expressed within the outflow tract (OT) and atrioventricular (AV) cushion cells at all stages examined, whereas TIMP-3 mRNA was undetectable in the AV cushion cells soon after their formation. Subsequently, TIMP-3 mRNA disappeared in cushion cells of the distal OT and this loss progressed toward the ventricle until eventually all of the OT cushion cells lacked detectable TIMP-3 transcripts. TIMP-3, but not TIMP-2, was also expressed within remodeling myocardium. Immunocytochemistry confirmed these findings. These observations suggest that TIMP-2 and TIMP-3 have important but unique roles in early cardiac development. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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