期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 99, 期 10, 页码 7113-7118出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.032062099
关键词
intercellular communication; channels; voltage gating; rectifaction; electrical coupling
资金
- NIGMS NIH HHS [R01 GM054179, GM54179] Funding Source: Medline
- NINDS NIH HHS [NS36706, R01 NS036706] Funding Source: Medline
Although fast and slow gating mechanisms have been described in gap junctions (GJs), their relative contributions to dependence on transjunctional voltage, V-j, is still unclear. We used cell lines expressing wild-type connexin 45 (Cx45) and connexin 43 fused with enhanced green fluorescent protein (Cx43-EGFP) to examine mechanisms of gating in homo- and heterotypic GJs formed of these connexins. Macroscopically Cx45/Cx45 channels show high sensitivity to V-j. Cx45 channels demonstrate two types of gating: fast transitions between open and residual states and slow transitions between open and completely closed states. Single-channel conductance of the Cx45 channel is approximate to32 pS for the open state and approximate to4 pS for the residual state. Cx45/Cx43-EGFP heterotypic junctions exhibit very asymmetrical V-j gating with the maximum junctional conductance shifted to V-j positive on the Cx45 side. Conductance of single Cx45/Cx43-EGFP channels is approximate to55 pS for the open state and approximate to4 pS for the residual state, values consistent with the simple-series connection of Cx45 and Cx43-EGFP hemichannels. At V-j = 0, the slow gate of many Cx45 hemichannels is closed in both homotypic Cx45/Cx45 and heterotypic Cx45/Cx43-EGFP junctions. Fast and slow V-j gates of both Cx45 and Cx43 hemichannels close for relative negativity at their cytoplasmic end. Coupling mediated by Cx45/Cx43-EGFP junctions can exhibit asymmetry that can be strongly modulated by small changes in difference of holding potentials. Cx45/Cx43 junctions are likely to be found in brain and heart and may mediate rectifying electrical transmission or modulatable chemical communication.
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