期刊
NUCLEIC ACIDS RESEARCH
卷 30, 期 10, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/30.10.e47
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资金
- Intramural NIH HHS [Z01 AG000767] Funding Source: Medline
- NCI NIH HHS [R29CA69421] Funding Source: Medline
- NIA NIH HHS [AG13478, K01 AG00767] Funding Source: Medline
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.
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