期刊
BIOELECTROCHEMISTRY
卷 56, 期 1-2, 页码 131-133出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/S1567-5394(02)00029-4
关键词
electropolymerization; biotinylated polypyrrole; impedance spectroscopy; immunosensor
The potentialities of an electrodeposited biotinylated polypyrrole film as an immobilisation matrix for the fabrication of impedimetric immunosensors are described. Biotinylated antibody (anti-human IgG), used as a model system, was attached to free biotin groups on the electrogenerated. polypyrrole film using avidin as a coupling reagent. This immobilization method allows to obtain a highly reproducible and stable device. The resulting immunosensor has a linear dynamic range of 10-80 ng ml(-1) of antigen and a detection limit of 10 pg ml(-1). Furthermore, this immunosensor exhibited minor loss in response after two regeneration steps. (C) 2002 Elsevier Science B.V All rights reserved.
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