期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 541, 期 1, 页码 159-167出版社
WILEY
DOI: 10.1113/jphysiol.2001.013268
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资金
- NHLBI NIH HHS [R37 HL042873, R01 HL042873, HL-42873] Funding Source: Medline
Intracellular H+ mobility was estimated in the rabbit isolated ventricular myocyte by diffusing HCl into the cell from a patch pipette, while imaging pH(i) confocally using intracellular ratiometric SNARF fluorescence. The delay for acid diffusion between two downstream regions similar to40 mum apart was reduced from similar to25 s to similar to6 s by replacing Hepes buffer in the extracellular superfusate with a 5% CO2/HCO3- buffer system (at constant pH(o) of 7.40). Thus CO2/HCO3- (carbonic) buffer facilitates apparent H-i(+) mobility. The delay with carbonic buffer was increased again by adding acetazolamide (ATZ), a membrane permeant carbonic anhydrase (CA) inhibitor. Thus facilitation of apparent H-i(+) mobility by CO2/HCO3- relies on the activity of intracellular CA. By using a mathematical model of diffusion, the apparent intracellular H+ equivalent diffusion coefficient (D-app(H)) in CO2/HCO3- buffered conditions was estimated to be 21.9 x 10(-7) cm(2) s(-1), 5.8 times faster than in the absence of carbonic buffer. Facilitation of H-i(+) mobility is discussed in terms of an intracellular carbonic buffer shuttle, catalysed by intracellular CA. Turnover of this shuttle is postulated to be faster than that of the intrinsic buffer shuttle. By regulating the carbonic shuttle, CA regulates effective H-i(+) mobility which, in turn, regulates the spatiotemporal uniformity of pH(i). This is postulated to be a major function of CA in heart.
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