期刊
AQUACULTURE
卷 207, 期 3-4, 页码 345-357出版社
ELSEVIER
DOI: 10.1016/S0044-8486(01)00792-X
关键词
detritus; fermentation; Undaria pinnatifida; hatchery diet; seaweed
Single cell detritus (SCD) is a product ca. 5.8-11.5 mum in diameter prepared by decomposing seaweed to a cellular level, and is expected to be utilized as a fisheries-diet in place of unicellular algae. The present paper details the culture conditions for the effective preparation of SCD from Undaria pinnatifida based on a fermentative method, The suitable NaCl concentration of the culture water for SCD-preparation was in the range of 2.5-3.5% (w/v) judged from the convenience for culture-treatment and the stability of the SCD-products. The temperature during culture did not make a marked difference on the productivity of the SCD in the range of 5-50 degreesC. As for the cellulase concentration, the addition of more than 1% was necessary to avoid rotting and to obtain a fermented culture. However, the addition of a microbial mixture composed of a lactic acid bacterium and two species of yeast at the start was effective to obtain a successfully fermented culture at a lower cellulase concentration of 0.25%. Under optimum conditions of culture containing 5% Undaria (on dry basis), 3.5% NaCl, 0.5% cellulase with an addition of the microbial mixture, SCD was produced at 5.8 x 10(7) cells ml(-1) after 6 days of incubation at 20 degreesC. The presently developed method needs no facilities for temperature conditioning, lightening, and air supplying, but needs only a plastic tank for obtaining the SCD. Here, we suggest a novel fisheries-diet of marine silage, which is produced from the combination of two principles, conversion of seaweed to SCD and induction of lactic acid fermentation utilizing activities of a microbial mixture composed of a lactic acid bacterium and yeast. (C) 2002 Elsevier Science B.V. All rights reserved.
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