期刊
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
卷 51, 期 3, 页码 273-281出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-022X(02)00029-5
关键词
CCD densitometry; PAGE; chlorophyll a/b ratio; light-harvesting complex; photosystem core
In this work, we present a home-made two-dimensional (2-D) CCD imaging system for the monochromatic densitometry of plane gels and its application to the imaging and densitometry of chlorophyll (Chl)-containing proteins separated by non-denaturing polyacrylamide gel electrophoresis. The monochromatic imaging of separated green bands at the wavelengths corresponding to their absorption band increases their contrast. This allows a better visualization of the faint-green bands in the gel and using of samples with lower Chl content for the electrophoresis. By the comparison of 2-D densitograms of the same gel illuminated with 670 and 650 nm lights, that is, at the red absorption maximum of Chl a and b, respectively, we achieved a selective imaging of the complexes with different Chl a/b ratio. This approach was used to specify an unknown band that appeared in the gel of the sample prepared from the thylakoid membranes of preheated barley leaves. (C) 2002 Elsevier Science B.V.. All rights reserved.
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