期刊
PLANT CELL AND ENVIRONMENT
卷 25, 期 6, 页码 815-819出版社
WILEY
DOI: 10.1046/j.1365-3040.2002.00863.x
关键词
Laurus nobilis L.; cavitation; centrifugation; embolism; water relations
A technique for measuring hydraulic conductances of excised xylem segments exposed to high negative pressures is described. A centrifugal force is used to generate negative pressures (P) in the sample and to create a positive hydrostatic pressure difference (DeltaP) between its two ends. DeltaP forces water through the sample at a flow rate (F) determined optically during centrifugation. The sample hydraulic conductance k is derived from F and DeltaP . The sample vulnerability curve is given by the dependence of k on P . Results for Cedrus atlantica Manetti and Laurus nobilis L. shoots are given. The technique is appropriate for the analysis of xylem refilling under negative pressure.
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