期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 50, 期 1, 页码 39-54出版社
ELSEVIER
DOI: 10.1016/S0167-7012(02)00013-1
关键词
Cr(VI); electron energy loss spectroscopy; environmental cell; microbial metal reduction; Shewanella oneidensis; transmission electron microscopy
Electron energy loss spectroscopy (EELS) techniques were used to determine oxidation state, at high spatial resolution. of chromium associated with the metal-reducing bacteria, Shewanella oneidensis, in anaerobic cultures containing Cr(VI)O-4(2-). These techniques were applied to fixed cells examined in thin section by conventional transmission electron microscopy (TEM) as well as unfixed, hydrated bacteria examined by environmental cell (EC)-TEM. Two distinct populations of bacteria were observed by TEM: bacteria exhibiting low image contrast and bacteria exhibiting high contrast in their cell membrane (or boundary) structure which was often encrusted with high-contrast precipitates. Measurements by EELS demonstrated that cell boundaries became saturated with low concentrations of Cr and the precipitates encrusting bacterial cells contained a reduced form of Cr in oxidation state +3 or lower. Published by Elsevier Science B.V.
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