Oxidant generation and toxicity enhancement of aged-diesel exhaust

Diesel exhaust related airborne Particulate Matter (PM) has been linked to a myriad of adverse health outcomes, ranging from cancer to cardiopulmonary disease. The underlying toxicological mechanisms are of great scientific interest. A hypothesis under investigation is that many of the adverse health effects may derive from oxidative stress, initiated by the formation of reactive oxygen species (ROS) within affected cells. In this study, the main objective was to determine whether aged-diesel exhaust PM has a higher oxidant generation and toxicity than fresh diesel exhaust PM. The diesel exhaust PM was generated from a 1980 Mercedes-Benz model 300SD, and a dual 270 m(3) Teflon film chamber was utilized to generate two test atmospheres. One side of the chamber is used to produce ozone-diesel exhaust PM system, and another side of the chamber was used to produce diesel exhaust PM only system. A newly optimized dithiothreitol (DTT) method was used to assess their oxidant generation and toxicity. The results Of this Study showed: (1) both fresh and aged-diesel exhaust PM had high oxidant generation and toxicity: (2) ozone-diesel exhaust PM had a higher toxicity response than diesel exhaust PM only: (3) the diesel exhaust PM toxicity increased with time; (4) the optimized DTT method could be used as a good quantitative chemical assay for oxidant generation and toxicity measurement. (C) 2008 Elsevier Ltd. All rights reserved.