期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 68, 期 6, 页码 3176-3179出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.68.6.3176-3179.2002
关键词
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The chitinase gene chiA was identified on the Clostridium thermocellum genome downstream of the endoglucanase gene celA. It contains a catalytic module of glycosyl hydrolase family 18 and a cellulosomal dockerin module. Chi18A hydrolyzes aryl-acetyl-chito-oligosaccharides preferentially. In denaturing electrophoresis of purified cellulosomes, a single chitinase activity band was identified in zymograms and Western blots, indicating that Chi18A is the only chitinase in the cellulosome.
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