期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 138, 期 3, 页码 199-206出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1047-8477(02)00036-9
关键词
chloramphenicol; Escherichia coli; nucleoid; toroid
The DNA of growing cells of Escherichia coli occurs in one or a few lobular bodies known as nucleoids. Upon exposure to chloramphenicol, the nucleoids assume compact, rounded forms (cm-nucleoids) that have been described as ring- or sphere-shaped. Multiple views of single cells or spheroplasts, however, support a different, curved toroid shape for cm-nucleoids. The multiple views were obtained either by DNA fluorescence imaging as the cells or spheroplasts reoriented in liquid medium or by optical sectioning using phase-contrast or fluorescence imaging of immobilized cells. The curved toroid shape is consistent with electron microscope images of thin sections of chloramphenicol-treated cells. The relationship of this structure to active and inactive nucleoids and to the smaller toroidal forms made by in vitro DNA condensation is discussed. Published by Elsevier Science (USA).
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