期刊
CANCER LETTERS
卷 180, 期 1, 页码 23-32出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/S0304-3835(02)00017-4
关键词
hepatocellular carcinoma; oncolytic recombinant adenovirus; alpha-fetoprotein; antitumoral effect
类别
Selectively replicating recombinant adenovirus has emerged as a novel strategy for the treatment of incurable human cancers. One of the major characteristics of hepatocellular carcinoma is the transcriptional reactivation of alpha-fetoprotein (AFP). In this study, we evaluated the liver cancer-specific oncolytic potential of E1B 55kDa-deleted recombinant adenovirus (YKL-1001), which retained other E I genes driven by the AFP promoter. Transient transfection study using luciferase indicated the selective activation of the AFP promoter only in human liver cancer cells secreting AFP (HepG2, Hep3B). YKL-1001 induced both cytopathic effects exclusively in AFP-positive liver cancer cells and the growth inhibition of pre-established Hep3B xenografts. Finally, hematoxylin-eosin staining and the immunohistochemistry to the adenoviral hexon showed a large distributed necrotic area and this implied a wide spread of YKL-1001. Therefore. the present study demonstrated that YKL-1001 holds significant promise as an oncolytic agent for hepatocellular carcinoma. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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