期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 17, 期 3-5, 页码 157-165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1381-1177(02)00023-1
关键词
biodiesel fuel; methanolysis; methyl ester; glutaraldehyde treatment; whole-cell biocatalyst
With a view to utilizing Rhizopus onzae cells immobilized within biomass support particles (BSPs) as a whole-cell biocatalyst for biodiesel fuel production, an investigation was made of the effect of cross-linking treatment with glutaraldehyde (GA) on the stability of lipase activity. Although the lipase activity of the BSP-immobilized cells decreased considerably in the presence of the methyl esters produced by methanolysis, the activity of cells treated with 0.1% GA solution showed no significant decrease during six batch cycles, with the methyl ester content of the reaction mixture reaching 70-83% in each cycle. In contrast, without GA treatment, activity decreased gradually with each cycle to give a methyl ester content of only 50% at the sixth batch cycle. These findings indicate that, given the simplicity of the lipase production process and the long-term stability of lipase activity, the use of whole-cell biocatalysts immobilized within BSPs and treated with GA solution offers a promising means of biodiesel fuel production for industrial application. (C) 2002 Elsevier Science B.V. All rights reserved.
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