期刊
CARBOHYDRATE RESEARCH
卷 337, 期 12, 页码 1119-1127出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0008-6215(02)00101-5
关键词
Shewanella putrefaciens; polysaccharide; NMR spectroscopy; nonulosonic acid; Shewanellose; biosynthesis
The structure of the phenol-soluble polysaccharide from Shewanella putrefaciens strain A6 has been elucidated. Chemical modifications of the polymer in conjunction with H-1 and C-13 NMR spectroscopy, including 2D techniques, were employed in the analysis. It is concluded that the repeating unit is composed of two nine-carbon sugars as follows: --> 4)-alpha-NonpA-(2 --> 3)-beta-Sugp-(1 --> where alpha-NonpA is 5-acetamido-7-acetamidino-8-O-acetyl-3,5,7,9-tetradeoxy-L-glycero-alpha-D-galacto-non-2-ulosonic acid (8eLeg) and beta-Sugp is 2-acetamido-2,6-dideoxy-4-C-(3'-carboxamide-2',2'-dihydroxypropyl)-beta-D-galactopyranose, with the proposed name Shewanellose (She). (C) 2002 Elsevier Science Ltd. All rights reserved.
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