期刊
PLANT SCIENCE
卷 163, 期 1, 页码 59-67出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/S0168-9452(02)00058-4
关键词
Fd-dependent-glutamine-2-oxoglutarate aminotransferase; glutamine synthetase; asparagine synthetase; glutamate dehydrogenase; diurnal changes in ammonia assimilation; transformed tobacco
The diurnal changes in carbon and nitrogen metabolite concentrations were studied in transgenic tobacco plants (Nicotiana tabacum L. cv. Xanthi) expressing an antisense ferredoxin-dependent glutamate synthase (Fd-GOGAT; EC 1.4.7.1) mRNA. In parallel, enzyme activities, and abundance of the proteins and transcripts involved in ammonia assimilation were monitored during the day/night cycle in both transgenic and untransformed control plants. When the transgenic plants were transferred from non-photorespiratory (high CO2) to photorespiratory conditions (air), photorespiratory NH4+ accumulated in the leaves during the second half of the light period; it decreased progressively through the night until the beginning of the next light period. Glutamine and 2-oxoglutarate (2-OG) also accumulated during the second part of the light period; this was followed by a decrease at the end of the night. A concomitant increase in asparagine was observed during the night, suggesting that this amino acid serves as a temporary storage compound for the partial elimination of excess photorespiratory ammonia. We also observed that the direction of the glutamate reaction varied during the day/night cycle such that a higher ratio of aminating/deaminating activity occurred in the first half of the light period. This was correlated with the decline in NH4+ and 2-OG concentrations. Both of these observations suggest that alternative metabolic pathways may be transiently activated during a day/night cycle in plants with low Fd-GOGAT activity. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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