期刊
PROCESS BIOCHEMISTRY
卷 37, 期 12, 页码 1375-1379出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0032-9592(02)00017-1
关键词
Ganoderma lucidum; ganoderic acid; higher fungi; polysaccharide; inoculation density; fermentation technology
Control of inoculation density was significant for cell growth, morphology, and production of polysaccharide and ganoderic acid in submerged culture of the higher fungus Ganoderma lucidum. A maximal cell concentration of 15.7 g dry cell weight (DW)/1 was obtained at an inoculation density of 330 mg DW/1. For inoculation density within the range of 70-670 mg DW/1, a large inoculation density led to a small pellet size and high production of extracellular and intracellular polysaccharides, while a relatively big pellet size and high accumulation of ganoderic acid were observed at a low inoculation density. It was also shown that small pellet size resulted in high polysaccharide production, while large pellet size led to high production of ganoderic acid. (C) 2002 Elsevier Science Ltd. All rights reserved.
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