期刊
GENES & DEVELOPMENT
卷 16, 期 13, 页码 1610-1615出版社
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.229402
关键词
Arabidopsis; embryo patterning; BODENLOS; MONOPTEROS; auxin response; root meristem initiation
Developmental responses to the plant hormone auxin are thought to be mediated by interacting pairs from two protein families: short-lived inhibitory IAA proteins and ARF transcription factors binding to auxin-response elements. monopteros mutants lacking activating ARF5 and the auxin-insensitive mutant bodenlos fail to initiate the root meristem during early embryogenesis. Here we show that the bodenlos phenotype results from an amino-acid exchange in the conserved degradation domain of IAA12. BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay and the two genes are coexpressed in early embryogenesis, suggesting that BODENLOS inhibits MONOPTEROS action in root meristem initiation.
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